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李晴

邮  箱: li.qing (AT) pku.edu.cn

职  称:教授

办公室地址:北京市海淀区颐和园路5号,北京大学,金光生命科学大楼,100871

所属实验室:李晴实验室

实验室电话:62759158

实验室地址:北京市海淀区颐和园路5号,北京大学,金光生命科学大楼,100871

  • 个人简介
  • 科研领域
  • 代表性论文
  • 实验室简介

教育经历:

2001 - 2006 , 理学博士 , 生物化学与分子生物学 , 北京大学
1997 - 2001 , 理学学士 , 生物化学与分子生物学 , 北京大学

工作经历:

2019.02 - 今   博雅特聘教授 北京大学生命科学学院
2018.02 - 2019.01 长聘副教授 北京大学生命科学学院
2012.01 - 2018.01 助理教授 北京大学生命科学学院
2012.01-今  研究员 北大清华生命科学联合中心
2006.09-2011.12 博士后 美国梅奥医学院癌症中心(Mayo Clinic Cancer Center)

社会服务工作:

2022-今 北京大学生命科学学院学术委员会委员
2022-今 北京大学生命科学学院学位委员会委员
2019-今 北京大学生命科学学院生物化学与分子生物学track(本科生),副主任
2018-今 蛋白质与植物基因研究国家重点实验室学术委员会 委员
2018-今 全国中学生英才计划生物委员会专家委员,中国科协
2018-今 北大-清华-NIBS研究生联合项目培养委员会组长(北大)

荣誉奖励:

2023 青年创新奖,中国遗传学会
2022 北京大学优秀研究生导师
2021 北京大学教学优秀奖-本科
2018 入选“北京高校卓越青年科学家”计划
2017 教育部青年科学奖
2017 国家杰出青年科学基金
2016 北京大学青年教师教学基本功比赛 理工科 二等奖
2015 郑昌学教学优秀奖
2014 拜耳学者奖
2013 国家优秀青年科学基金
2009 Mayo Clinic“Kendall-Mayo 奖学金”

学术任职:

2019-今 中国遗传学会表观遗传专业委员会副主任委员
2020-今 中国细胞生物学会染色质生物学分会委员
2019-今 中国生物化学与分子生物学会基因专业分会委员    
2018-今 中国病理生理学会系统生物医学专业委员会委员

执教课程:

分子生物学(本,主讲)
表观遗传学基础(本,主讲)
综合实验课(III)(本)
CLS生物化学与分子生物学模块(研)
现代生物学实验技术原理及其应用(研)
         表观遗传信息的继承是生命个体正常发育的基础,在生殖、发育和衰老等过程中发挥关键作用。该过程的失调与多种疾病相关,包括肿瘤、神经退行性病变和早衰等,因而对人类健康产生深远的影响。表观遗传信息的继承的核心阶段发生在细胞周期的S期中的DNA复制过程。伴随DNA复制,母链染色质需要解组装,而在子链上需要重建染色质,意味着母链表观遗传信息在子链间的分配和继承,子代细胞可弹性继承亲本细胞的可遗传的表观遗传信息。
  本实验室长期致力于表观遗传信息继承(Epigenetic Inheritance)的分子机制。我们以芽殖酵母和胚胎干细胞为研究模式,运用经典的生物化学、遗传学等方法结合前沿的组学方法、荧光成像等技术,以染色质复制过程为切入点,揭示表观遗传信息继承的基本规律,解析其与基因组稳定性维持,细胞命运决定等的关联机理。主要研究内容包括:
   1.染色质携带的表观遗传信息的继承和分配机制:重点关注“新、旧”组蛋白在DNA复制叉两条子链的传递机制,阐明该过程对异染色质的建立和维持的影响,探讨其在胚胎发育中的重要作用。
   2.染色质组装机器(包括组蛋白分子伴侣、染色质重塑因子和染色质修饰酶等调节因子)和DNA复制机器(复制体)的“对话”机制:DNA复制和染色质复制偶联是生命科学的基础问题之一,这是维持基因组稳定和表观遗传组稳定的核心基础,因此我们长期关注这两个基本过程的偶联机制,重点关注染色质组装对DNA复制反馈的调节机制,以及此过程异常和人类疾病的关系。
1. Ningning Li#, Yuan Gao#, Yujie Zhang#, Daqi Yu#, Jianwei Lin, Jianxun Feng, Jian Li, Zhichun Xu, Yingyi Zhang, Shangyu Dang, Keda Zhou, Yang Liu, Xiang David Li, Bik Kwoon Tye*, Qing Li*, Ning Gao*, and Yuanliang Zhai*. Parental histone transfer caught at the replication fork, Nature, 2024, https://doi.org/10.1038/s41586-024-07152-2
2. Hang Zhao#, Di Li#, Xue Xiao#, Cuifang Liu, Guifang Chen, Xiaoyu Su, Zhenxin Yan, Shijia Gu, Yizhou Wang, Guohong Li, Jianxun Feng, Wei Li, Ping Chen*, Jiayi Yang*, Qing Li*. Pluripotency state transition of embryonic stem cells requires the turnover of histone chaperone FACT on chromatin, iScience, 2024, 27(1): 108537.
3. Yang Liu#, Zhengrong Zhangding#, Xuhao Liu#, Tingting Gan#, Chen Ai, Jinchun Wu, Haoxin Liang, Mohan Chen, Yuefeng Guo, Rusen Lu, Yongpeng Jiang, Xiong Ji, Ning Gao, Daochun Kong, Qing Li, Jiazhi Hu*. Fork coupling directs DNA replication elongation and termination, Science, 2024, 383(6688): 1215-1222
4. Xuezheng Wang#, Yuantao Tang#, Jiawei Xu, He Leng, Guojun Shi, Zaifeng Hu, Jiale Wu, Yuwen Xiu, Jianxun Feng*, Qing Li*. The N-terminus of Spt16 anchors FACT to MCM2–7 for parental histone recycling, Nucleic Acids Research, 2023, 51(21): 11549-11567.
5. Wenlong Du#, Guojun Shi#, Chun-Min Shan#, Zhiming Li#, Bing Zhu*, Songtao Jia*, Qing Li*, Zhiguo Zhang*.(2022)Mechanisms of chromatin-based epigenetic inheritance. Science China Life Sciences, 65,https://doi.org/10.1007/s11427-022-2120-1 (Review)
6. He Leng#, Shaofeng Liu#, Yang Lei, Yuantao Tang, Shijia Gu, Jiazhi Hu, She Chen, Jianxun Feng *, Qing Li*. (2021) FACT interacts with Set3 HDAC and fine-tunes GAL1 transcription in response to environmental stimulation. Nucleic Acids Research, 49:5502-5519
7. Xinlin Xu#, Yixi Xu#*, Ruiyuan Guo,Ran Xu ,Congcong Fu, Mengtan Xing, Hiroyuki Sasanuma, Qing Li, Minoru Takata, Shunichi Takeda, Rong Guo *, Dongyi Xu *. (2021) Fanconi anemia proteins participate in a break-induced-replication-like pathway to counter replication stress. Nature Structural & Molecular Biology, 28:487–500
8. Yang Liu#, Chen Ai#, Tingting Gan#, Jinchun Wu, Yongpeng Jiang, Xuhao Liu, Rusen Lu, Ning Gao,Qing Li, Xiong Ji, Jiazhi Hu *.(2021) Transcription shapes DNA replication initiation to preserve genome integrity. Genome Biology, 22:176
9. Haoyang Peng#, Simin Zhang#, Yihan Peng#, Shuangyi Zhu, Xin Zhao, Xiaocong Zhao, Shuangshuang Yang, Guangxue Liu, Yang Dong, Xiaoli Gan, Qing Li, Xinghua Zhang, Huadong Pei*, Xuefeng Chen*.(2021) Yeast Bromodomain Factor 1 and Its Human Homolog TAF1 Play Conserved Roles in Promoting Homologous Recombination. Advanced Science, 2100753
10. Zhiyun Xu#, Jianxun Feng* , Qing Li*. (2021) Measuring Genome-Wide Nascent Nucleosome Assembly Using Replication-intermediate nucleosome mapping (ReIN-Map). Methods Molecular Biology (Book) 2196:117-141. doi: 10.1007/978-1-0716-0868-5_10. PMID: 32889717.
11. Tian Tian#, Min Bu#, Xu Chen#,Linli Ding#, Yulan Yang#, Jinhua Han, Xin-Hua Feng, Pinglong Xu, Ting Liu, Songmin Ying, Yang Lei, Qing Li, Jun Huang*. (2021) The ZATT-TOP2A-PICH axis drives extensive replication fork reversal to promote genome stability. Molecular Cell 81: 198-211.e6.
12. Yutong Zhang#, Xiaomin Ou#, Xuezheng Wang , Dongjie Sun, Xueyin Zhou, Xiaofei Wu, Qing Li, Long Li*. (2020)Structure of the mitochondrial TIM22 complex from yeast. Cell Research, https://doi.org/10.1038/s41422-020-00399-0.
13. Yi Ching Esther Wan#,Tsz Chui Sophia Leung#, Dongbo Ding#, Xulun Sun, Jiaxian Liu,Lina Zhu, Tze Zhen Evangeline Kang, Du Yang, Yuchen Zhang, Jitian Zhang, Chengmin Qian, Michael Shing Yan Huen, Qing Li, Maggie Zi Ying Chow, Zongli Zheng, Junhong Han, Ajay Goel, Xin Wang*, Toyotaka Ishibashi*, Kui Ming Chan*. (2020) Cancer-associated histone mutation H2BG53D disrupts DNA-histone octamer interaction and promotes oncogenic phenotypes. Signal Transduction and Targeted Therapy, 5(1):27 https://doi.org/10.1038/s41392-020-0131-0.
14. Shuqi Li #, Ziqi Dong #, Shuangshuang Yang, Jianxun Feng, Qing Li *. (2019) Chaperoning RPA during DNA metabolism. Current Genetics, 65: 857-864. (Review)
15. Qing Li*, Xu Zhang, Zhiguo Zhang*. (2018). CHAF1B overexpression: A brake for the differentiation of leukemia cells. Cancer Cell 34:693-694. (Preview)
16. Shuqi Li#, Zhiyun Xu #, Jiawei Xu #, Linyu Zuo, Chuanhe Yu,Pu Zheng, Haiyun Gan, Xuezheng Wang, Longtu Li, Sushma Sharma, Andrei Chabes, Di Li, Sheng Wang, Sihao Zheng, Jinbao Li, Xuefeng Chen, Yujie Sun, Dongyi Xu, Junhong Han, Kuiming Chan, Zhi Qi , Jianxun Feng.*, Qing Li*. (2018) Rtt105 functions as a chaperone for replication protein A to preserve genome stability. The EMBO Journal e99154.
17. Xiaowei Yan#, Jiayi Yang#, Jiawei Xu#,Jianxun Feng*, Qing Li*. (2018). Histone chaperone Spt16p is required for heterochromatin mediated silencing in budding yeast. Protein & Cell 9:652-658.
18. Shengxian Gao, Sumin Feng, Shaokai Ning, Jingyan Liu, Huayu Zhao, Yixi Xu, Jinfeng Shang, Kejiao Li, Qing Li, Rong Guo, Dongyi Xu. (2018) An OB-fold complex controls the repair pathways for DNA double-strand breaks. Nature Communications 9: 3925.
19. Shaofeng Liu#, Zhiyun Xu#,He Leng#, Pu Zheng, Jiayi Yang,Kaifu Chen, Jianxun Feng, Qing Li *. (2017). RPA binds histone H3-H4 and functions in DNA replication-coupled nucleosome assembly. Science 355, 415-420.
20. Wei Li#, Ping Chen#, Juan Yu, Liping Dong, Dan Liang, Jianxun Feng, Jie Yan, Peng-Ye Wang, Qing Li, Zhiguo Zhang, Ming Li*, Guohong Li*. (2016). FACT remodels the tetranucleosomal unit of chromatin fibers for gene transcription. Molecular Cell 64, 120-133.
21. Jianxun Feng#, Haiyun Gan#, Matthew L Eaton, Hui Zhou, Shuqi Li, Jason A Belsky, David M MacAlpine, Zhiguo Zhang*, Qing Li*. (2016). Noncoding transcription is a driving force for nucleosome instability in spt16 mutant cells. Molecular and Cellular Biology 36, 1856-1867. Article of significant interest selected as a Spotlight.
22. Jiayi Yang#, Xu Zhang#, Jianxun Feng#,He Leng, Shuqi Li, Junyu Xiao,Shaofeng Liu, Zhiyun Xu, Jiawei Xu, Di Li, Zhongshi Wang, Jingyang Wang, and Qing Li*. (2016). The Histone Chaperone FACT Contributes to DNA Replication-Coupled Nucleosome Assembly. Cell Reports 14, 1128-1141.
23. Zhang Xu and Qing Li*. (2014). A role of histone modifications during chromatin replication. Chinese Bulletin of Life Sciences 26, 1176-1186. (In Chinese) (Review)
24. Dan Su#, Qi Hu#, Qing Li#, James R Thompson, Gaofeng Cui, Ahmed Fazly, Brian A Davies, Maria Victoria Botuyan, Zhiguo Zhang* and Georges Mer*. (2012) Structural basis for recognition of H3K56-acetylated histone H3–H4 by the chaperone Rtt106. Nature 483, 104-107.
25. Qing Li#, Rebecca Burgess#and Zhiguo Zhang*. (2012). All roads lead to chromatin: Multiple pathways for histone deposition. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. 1819, 238-46.
26. Qing Li and Zhiguo Zhang* .(2012) Linking DNA replication to heterochromatin silencing and epigenetic inheritance. Acta Biochimica et Biophysica Sinica (ABBS) 44, 3-13. This review was selected as the cover story.
27. Ahmed Fazly, Qing Li, Qi Hu, Georges Mer, Bruce Horazdovsky, and Zhiguo Zhang* .(2012). Histone chaperone Rtt106 promotes nucleosome formation using (H3-H4)2 tetramers. The Journal of Biological Chemistry 2287, 10753-10760.
28. Rebecca J Burgess, Hui Zhou, Junhong Han, Qing Li, and Zhiguo Zhang*. (2012). The SCFDia2 ubiquitin E3 ligase ubiquitylates Sir4 and functions in transcriptional silencing. PLoS Genetics 8, e1002846
29. Junhong Han, Qing Li, Laura McCullough, Charisse Kettelkamp, Tim Formosa, and Zhiguo Zhang* .(2010) Ubiquitylation of FACT by the Cullin-E3 ligase Rtt101 connects FACT to DNA replication. Genes & Development 24, 1485-1490.
30. Qing Li, A M Fazly, Hui Zhou, Shengbing Huang, Zhiguo Zhang* and Bruce Stillman*.(2009) The Elongator complex interacts with PCNA and modulates transcriptional silencing and sensitivity to DNA damage agents. PLoS Genetics 5, e1000684.
31. Qing Li#, Hui Zhou#, Hugo Wurtele, Brian Davies, Bruce Horazdovsky, Alain Verreault*, and Zhiguo Zhang*. (2008) Acetylation of Histone H3 lysine 56 regulates replication-coupled nucleosome assembly. Cell 134, 244-255. Previewed in the same issue. Highlighted in Nature Reviews Molecular Cell Biology, September 2008
32. Pei Han#, Qing Li#, and Yu-Xian Zhu*.(2008) Mutation of Arabidopsis BARD1 Causes Meristem Defects by Failing to Confine WUSCHEL Expression to the Organizing Center. The Plant Cell 20, 1482-93.
33. Qing Li#, Bai-Chen Wang, Yu Xu, and Yu-Xian Zhu*. (2007) Systematic studies of 12S seed storage protein accumulation and degradation patterns during Arabidopsis seed maturation and early seedling germination stages. Journal of Biochemistry and Molecular Biology 40, 373-381.
34. Qing Li, Jian-Xun Feng, Pei Han, and Yu-Xian Zhu*. (2006) Parental RNA is significantly degraded during Arabidopsis seed germination. Journal of Integrative Plant Biology 48, 114-120.
李晴实验室

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