生命科学学院学术报告
题目：Investigating Breast Tumor Evolution with Single Cell DNA Sequencing
演讲人：Yong Wang，Ph.D.
Assistant Professor,
Dept. of Medicine, Univ. of Wisconsin-Madison
时间：2018年5月24日 13:30-14:30
地点：金光生命科学大楼411
摘要：
To further advance personalized cancer therapy, a more comprehensive understanding of intratumor heterogeneity is required.  Dr. Yong Wang developed a single cell DNA sequencing technology, applied the technology to investigate intratumor heterogeneity in breast cancer patients and detected large number of rare subclonal mutations that may play important roles in diversifying the phenotypes of cancer cells. By profiling hundreds of individual tumor and normal cells, Yong found that point mutations evolved gradually over long periods of time, generating extensive clonal diversity, while large scale chromosome rearrangements occurred early, in punctuated bursts of evolution, followed by stable clonal expansions to form the tumor mass. However, the data resulting from these studies was still challenged by technical errors. To address this problem, Yong developed a statistical method called Monovar, which uses a genotype likelihood model and multi-cell variant calling approach to mitigate these errors. Yong is currently developing his own research project that investigates tissue mosaicism and tumor initiation in breast cancer patients. His preliminary data suggests that tissue mosaic mutations may be common in normal breast tissues and mosaic mutations that occurred in oncogenes or tumor suppressor genes may lead to tumor initiation. These results may have significant implications in cancer prevention and early detection by identifying genetic factors that initiate tumor growth. The technologies and methods that he developed will find broad applications in many diverse research fields and clinical settings.
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