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汤富酬
邮  箱: tangfuchou(AT)pku.edu.cn
职  称:
研究员
办公室地址: 北京市海淀区颐和园路5号,北京大学,综合科研楼,100871
实验室地址: 北京市海淀区颐和园路5号,北京大学,综合科研楼,100871
个人简历
教育经历
1994 - 1998    本科, 遗传学, 北京大学
1998 - 2003    博士, 细胞生物学, 北京大学
工作经历
2004 - 2010     英国剑桥大学Gurdon研究所,博士后
2010 - 现在     研究员, 北京大学生命科学学院生物动态光学成像中心
2015 - 现在     研究员, 北大-清华生命科学联合中心
荣誉奖励
2014年度中国科学十大进展(“利用极体高通量测序结果精确推演出母源基因组信息”)
2015年度中国科学十大进展(“揭示人类原始生殖细胞基因表达与表观遗传调控特征”)
2015年度中国生命科学领域十大进展
杂志编辑
2014 - 至今  《Science Bulletin》(《科学通报》英文版)编委(Editorial Board Member)
2015 - 至今  《Genome Biology》编委(Editorial Board Member)
2016 - 至今  《Open Biology》编委(Editorial Board Member)
科研领域描述
具有自我更新能力和分化潜能的干细胞是哺乳动物胚胎发育过程中以及成体中的关键类型的细胞,对各种干细胞进行深入研究是理解哺乳动物发育、生长机制的关键,也是将干细胞应用于临床再生医学、治疗人类疾病的前提。本实验室主要围绕人类早期胚胎发育研究多能干细胞的自我更新和多能性调控的分子机理,特别是表观遗传学调控机理,以及相关的早期生殖细胞发育过程中的表观遗传学重编程机理。利用我们发展的单细胞功能基因组学分析技术(单细胞转录组测序技术、单细胞 DNA甲基化组测序技术、单细胞染色质状态组测序技术、单细胞多组学平行测序技术等),以及基因编辑技术、染色质免疫共沉淀-高通量测序技术、小鼠胚胎显微操作技术、以及人类胚胎干细胞体外定向分化等技术在单细胞和单碱基分辨率深入分析人类早期胚胎、生殖系细胞、以及多能性干细胞中基因表达网络的表观遗传学调控机理。
代表性论文
1. Liu J, Liu W, Yang L, Wu Q, Zhang H, Fang A, Li L, Xu X, Sun L, Zhang J*, Tang Fuchou*, Wang X*. The Primate-Specific Gene TMEM14B Marks Outer Radial Glia Cells and Promotes Cortical Expansion and Folding. Cell Stem Cell, 2017, doi.org/10.1016/j.stem.2017.08.013 (*: Co-corresponding authors).

2. Wen L* & Tang Fuchou*. Single cell epigenome sequencing technologies. Molecular Aspects of Medicine, 2017 (Review) (*: Co-corresponding authors).

3. Yang J, Li J, Suzuki K, Liu X, Wu J, Zhang W, Ren R, Zhang W, Chan P, Izpisua Belmonte JC, Qu J*, Tang Fuchou*, Liu GH*. Genetic enhancement in cultured human adult stem cells conferred by a single nucleotide recoding. Cell Research, 2017, 27: 1178-1181 (*: Co-corresponding authors).

4. Guo F*, Li L, Li J, Wu X, Hu B, Zhu P, Wen L, Tang Fuchou*. Single-cell multi-omics sequencing of mouse early embryos and embryonic stem cells. Cell Research, 2017, 27: 967-988 (*: Co-corresponding authors).

5. Li L, Dong J, Yan L, Yong J, Liu X, Hu Y, Fan X, Wu X, Guo H, Wang X, Zhu X, Li R, Yan J, Wei Y, Zhao Y, Wang W, Ren Y, Yuan P, Yan Z, Hu B, Guo F, Wen L, Tang Fuchou*, Qiao J*. Single-Cell RNA-Seq Analysis Maps Development of Human Germline Cells and Gonadal Niche Interactions. Cell Stem Cell, 2017, 20: 858-873 (*: Co-corresponding authors).

6. Zhu C, Gao Y, Guo H, Xia B, Song J, Wu X, Zeng H, Kee K, Tang Fuchou*, Yi C*. Single-Cell 5-Formylcytosine Landscapes of Mammalian Early Embryos and ESCs at Single-Base Resolution. Cell Stem Cell, 2017, 20: 720-731 (*: Co-corresponding authors).

7. Guo H, Hu B, Yan L, Yong J, Wu Y, Gao Y, Guo F, Hou Y, Fan X, Dong J, Wang X, Zhu X, Yan J, Wei Y, Jin H, Zhang W, Wen L, Tang Fuchou*, Qiao J*. DNA methylation and chromatin accessibility profiling of mouse and human fetal germ cells. Cell Research, 2017, 27: 165-183 (*: Co-corresponding authors).

8. Yang L, Ma Z, Cao C, Zhang Y, Wu X, Lee R, Hu B, Wen L, Ge H, Huang Y*, Lao K*, Tang Fuchou*. MR-seq: measuring a single cell’s transcriptome repeatedly by RNA-seq. Science Bulletin, 2017, 62: 391-398 (*: Co-corresponding authors).

9. Zhou F, Li X, Wang W, Zhu P, Zhou J, He W, Ding M, Xiong F, Zheng X, Li Z, Ni Y, Mu X, Wen L, Cheng T, Lan Y, Yuan W*, Tang Fuchou*, Liu B*. Tracing haematopoietic stem cell formation at single-cell resolution. Nature, 2016, 533: 487-492 (*: Co-corresponding authors).

10. Dang Y, Yan L, Hu B, Fan X, Ren Y, Li R, Lian Y, Yan J, Li Q, Zhang Y, Li M, Ren X, Huang J, Wu Y, Liu P, Wen L, Zhang C, Huang Y*, Tang Fuchou*, Qiao J*. Tracing the expression of circular RNAs in human pre-implantation embryos. Genome Biology, 2016, 17: 130 (*: Co-corresponding authors).

11. Wen L*, Tang Fuchou*. Single-cell sequencing in stem cell biology. Genome Biology, 2016, 17: 71 (Review) (*: Co-corresponding authors).

12. Hou Y, Guo H, Cao C, Li X, Hu B, Zhu P, Wu X, Wen L, Tang Fuchou*, Huang Y*, Peng J*. Single-cell triple omics sequencing reveals genetic, epigenetic, and transcriptomic heterogeneity in hepatocellular carcinomas. Cell Research, 2016, 26: 304-319 (*: Co-corresponding authors).

13. Pan H, Guan D, Liu X, Li J, Wang L, Wu J, Zhou J, Zhang W, Ren R, Zhang W, Li Y, Yang J, Hao Y, Yuan T, Yuan G, Wang H, Ju Z, Mao Z, Li J, Qu J*, Tang Fuchou*, Liu GH*. SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2. Cell Research, 2016, 26: 190-205 (*: Co-corresponding authors).

14. Yan L, Guo H, Hu B, Li R, Yong J, Zhao Y, Zhi X, Fan X, Guo F, Wang X, Wang W, Wei Y, Wang Y, Wen L, Qiao J*, Tang Fuchou*. Epigenomic landscape of human fetal brain, heart, and liver. Journal of Biological Chemistry, 2016, 291: 4386-4398 (*: Co-corresponding authors).

15. Wen L*, Tang Fuchou*, How to catch rare cell types. Nature, 2015, 525: 197-198 (Preview) (*: Co-corresponding authors).

16. Guo F, Yan L, Guo H, Li L, Hu B, Zhao Y, Yong J, Hu Y, Wang X, Wei Y, Wang W, Li R, Yan J, Zhi X, Zhang Y, Jin H, Zhang W, Hou Y, Zhu P, Li J, Zhang L, Liu S, Ren Y, Zhu X, Wen L, Gao YQ, Tang Fuchou*, Qiao J*. The transcriptome and DNA methylome landscapes of human primordial germ cells. Cell, 2015, 161: 1437-1452 (*: Co-corresponding authors).

17. Zhang W, Li J, Suzuki K, Qu J, Wang P, Zhou J, Liu X, Ren R, Xu X, Ocampo A, Yuan T, Yang J, Li Y, Shi L, Guan D, Pan H, Duan S, Ding Z, Li M, Yi F, Bai R, Wang Y, Chen C, Yang F, Li X, Wang Z, Aizawa E, Goebl A, Soligalla RD, Reddy P, Esteban CR, Tang Fuchou*, Liu GH*, Belmonte JC*. A Werner syndrome stem cell model unveils heterochromatin alterations as a driver of human aging. Science, 2015, 348: 1160-1163 (*: Co-corresponding authors).

18. Wen L*, Tang Fuchou*, Charting a Map through the Cellular Reprogramming Landscape. Cell Stem Cell, 2015, 16: 215-216 (Preview) (*: Co-corresponding authors).

19. Yan L, Huang L, Xu L, Huang J, Ma F, Zhu X, Tang Y, Liu M, Lian Y, Liu P, Li R, Lu S, Tang Fuchou*, Qiao J*, Xie XS*. Live births after simultaneous avoidance of monogenic diseases and chromosome abnormality by next-generation sequencing with linkage analyses. Proc Natl Acad Sci U S A, 2015, 112: 15964-15969 (*: Co-corresponding authors).

20. Duan S, Yuan G, Liu X, Ren R, Li J, Zhang W, Wu J, Xu X, Fu L, Li Y, Yang J, Zhang W, Bai R, Yi F, Suzuki K, Gao H, Esteban CR, Zhang C, Izpisua Belmonte JC, Chen Z, Wang X, Jiang T, Qu J*, Tang Fuchou*, Liu GH*. PTEN deficiency reprogrammes human neural stem cells towards a glioblastoma stem cell-like phenotype. Nature Communications, 2015, 6: 10068 (*: Co-corresponding authors).

21. Wen L, Li J, Guo H, Liu X, Zheng S, Zhang D, Zhu W, Qu J, Guo L, Du D, Jin X, Zhang Y, Gao Y, Shen J, Ge H, Tang Fuchou*, Huang Y*, Peng J*. Genome-scale detection of hypermethylated CpG islands in circulating cell-free DNA of hepatocellular carcinoma patients. Cell Research, 2015, 25: 1250-1264 (*: Co-corresponding authors).

22. Fan X, Zhang X, Wu X, Guo H, Hu Y, Tang Fuchou*, Huang Y*. Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos. Genome Biology, 2015, 16: 148 (*: Co-corresponding authors)

23. Guo H, Zhu P, Guo F, Li X, Wu X, Fan X, Wen L*, Tang Fuchou*. Profiling DNA methylome landscapes of mammalian cells with single-cell reduced-representation bisulfite sequencing. Nature Protocols, 2015, 10: 645-659 (*: Co-corresponding authors).

24. Wu Y, Zhou H, Fan X, Zhang Y, Zhang M, Wang Y, Xie Z, Bai M, Yin Q, Liang D, Tang W, Liao J, Zhou C, Liu W, Zhu P, Guo H, Pan H, Wu C, Shi H, Wu L* , Tang Fuchou*, Li J,*. Correction of a Genetic Disease by CRISPR-Cas9-Mediated Gene Editing in Mouse Spermatogonial Stem Cells. Cell Research , 2015 , 25: 67-79 (*: Co-corresponding authors).

25. Shen J, Jiang D, Fu Y, Wu X, Guo H, Feng B, Pang Y, Streets AM, Tang Fuchou*, Huang Y*. H3K4me3 epigenomic landscape derived from ChIP-Seq of 1 000 mouse early embryonic cells. Cell Research, 2015, 25: 143-147 (*: Co-corresponding authors).

26. Guo F, Li X, Liang D, Li T, Zhu P, Guo H, Wu X, Wen L, Gu TP, Hu B, Walsh CP, Li J*, Tang Fuchou*, Xu GL*. Active and Passive Demethylation of Male and Female Pronuclear DNA in the Mammalian Zygote. Cell Stem Cell, 2014, 15: 447-458 (*: Co-corresponding authors).

27. Guo H, Zhu P, Yan L, Li R, Hu B, Lian Y, Yan J, Ren X, Lin S, Li J, Jin X, Shi X, Liu P, Wang X, Wang W, Wei Y, Li X, Guo F, Wu X, Fan X, Yong J, Wen L, Xie SX, Tang Fuchou*, Qiao J*. The DNA methylation landscape of human early embryos. Nature, 2014, 511: 606-610 (*: Co-corresponding authors).

28. Wen L, Tang Fuchou*, Reconstructing Complex Tissues from Single-Cell Analyses. Cell, 2014, 157: 771-773 (*: Corresponding author).

29. Wen L, Li X, Yan L, Tan Y, Li R, Zhao Y, Wang Y, Xie J, Zhang Y, Song C, Yu M, Liu X, Zhu P, Li X, Hou Y, Guo H, Wu X, He C*, Li R*, Tang Fuchou*, Qiao J*. Whole-genome analysis of 5-hydroxymethylcytosine and 5-methylcytosine at base resolution in the human brain. Genome Biology, 2014, 15: R49 (*: Co-corresponding authors).

30. Streets AM, Zhang X, Cao C, Pang Y, Wu X, Xiong L, Yang L, Fu Y, Zhao L*, Tang Fuchou*, Huang Y*. Microfluidic single-cell whole-transcriptome sequencing. Proc Natl Acad Sci U S A, 2014, 111: 7048-7053 (*: Co-corresponding authors).

31. Hou Y, Fan W, Yan L, Li R, Lian Y, Huang J, Li J, Xu L, Tang Fuchou*, Xie XS*, Qiao J*. Genome Analyses of Single Human Oocytes. Cell, 2013, 155: 1492-1506 (*: Co-corresponding authors).

32. Guo H, Zhu P, Wu X, Li X, Wen L, Tang Fuchou*. Single-cell methylome landscapes of mouse embryonic stem cells and early embryos analyzed using reduced representation bisulfite sequencing. Genome Research, 2013, 23: 2126-2135 (*: Corresponding author).

33. Yan L, Yang M, Guo H, Yang L, Wu J, Li R, Liu P, Lian Y, Zheng X, Yan J, Huang J, Li M, Wu X, Wen L, Lao K, Li R*, Qiao J*, Tang Fuchou*. Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells. Nature Structural & Molecular Biology, 2013, 20: 1131-1139 (*: Co-corresponding authors).

34. Gan H, Wen L, Liao S, Lin X, Ma T, Liu J, Song CX, Wang M, He C, Han C*, Tang Fuchou*. Dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis. Nature Communications, 2013, 4: 1995 (*: Co-corresponding authors).

35. Hu X, Zhang L, Mao SQ, Li Z, Chen J, Zhang RR, Wu HP, Gao J, Guo F, Liu W, Xu GF, Dai HQ, Shi YG, Li X, Hu B, Tang F., Pei D, Xu GL. Tet and TDG Mediate DNA Demethylation Essential for Mesenchymal-to-Epithelial Transition in Somatic Cell Reprogramming. Cell Stem Cell, 2014, 14: 512-522.

36. Grabole N, Tischler J, Hackett JA, Kim S, Tang F., Leitch HG, Magnúsdóttir E, Surani MA. Prdm14 promotes germline fate and naive pluripotency by repressing FGF signalling and DNA methylation. EMBO Reports, 2013, 14: 629-637.

37. Zhang RR, Cui QY, Murai K, Lim YC, Smith ZD, Jin S, Ye P, Rosa L, Lee YK, Wu HP, Liu W, Xu ZM, Yang L, Ding YQ, Tang F. Meissner A, Ding C, Shi Y, Xu GL. Tet1 Regulates Adult Hippocampal Neurogenesis and Cognition. Cell Stem Cell, 2013, 13: 237-245.

38. Magnúsdóttir E, Dietmann S, Murakami K, Günesdogan U, Tang F., Bao S, Diamanti E, Lao K, Gottgens B, Surani MA. A tripartite transcription factor network regulates primordial germ cell specification in mice. Nature Cell Biology, 2013, 15: 905-915.

39. Lu S, Zong C, Fan W, Yang M, Li J, Chapman AR, Zhu P, Hu X, Xu L, Yan L, Bai F, Qiao J, Tang F., Li R, Xie XS. Probing meiotic recombination and aneuploidy of single sperm cells by whole-genome sequencing, Science, 2012, 338: 1627-1630.

40. Bao S, Leitch HG, Gillich A, Nichols J, Tang F., Kim S, Lee C, Zwaka T, Li X, Surani MA, The germ cell determinant blimp1 is not required for derivation of pluripotent stem cells. Cell Stem Cell, 2012, 11: 110-117.

41. Tang F, Lao K, Surani MA. Development and applications of single-cell transcriptome analysis. Nature Methods, 2011, 8: S6-S11 (Review).

42. Tang F, Barbacioru C, Bao S, Lee C, Nordman E, Wang X, Lao K, Surani MA. Tracing the Derivation of Embryonic Stem Cells from the Inner Cell Mass by Single-Cell RNA-Seq Analysis. Cell Stem Cell, 2010, 6: 468 – 478.

43. Tang Fuchou*. Small RNAs in mammalian germline: tiny for immortal. Differentiation, 2010, 79: 141-146 (Review) (*: Corresponding author)  

44. Tang F, Barbacioru C, Nordman E, Li B, Xu N, Bashkirov VI, Lao K, Surani MA. RNA-Seq analysis to capture the transcriptome landscape of a single cell. Nature Protocols , 2010, 5: 516-535.

45. Bao S*, Tang F*, Li X, Hayashi K, Gillich A, Lao K, Surani MA. Epigenetic reversion of postimplantation epiblast to pluripotent embryonic stem cells. Nature, 2009, 461: 1292-1295 (*: co-first authors).

46. Tang F*, Barbacioru C*, Wang Y, Nordman E, Lee C, Xu N, Wang X, Bodeau J, Tuch BB, Siddiqui A, Lao K, Surani MA. mRNA-Seq whole transcriptome analysis of a single cell. Nature Methods, 2009, 6: 377-382 (*: co-first authors).

47. Hayashi K, Lopes SM, Tang F, Surani MA. Dynamic equilibrium and heterogeneity of mouse pluripotent stem cells with distinct functional and epigenetic states. Cell Stem Cell, 2008, 3: 391-401.

48. Tang F*, Kaneda M*, O'Carroll D, Hajkova P, Barton SC, Sun YA, Lee C, Tarakhovsky A, Lao K, Surani MA. Maternal microRNAs are essential for mouse zygotic development. Genes & Development, 2007, 21: 644-648 (*: co-first authors).

49. Tang F, Hajkova P, Barton SC, O'Carroll D, Lee C, Lao K, Surani MA. 220-plex microRNA expression profile of a single cell. Nature Protocols, 2006, 1: 1154-1159.

50. Tang F, Hajkova P, Barton SC, Lao K, Surani MA. MicroRNA expression profiling of single whole embryonic stem cells. Nucleic Acids Research, 2006, 34: e9.
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