60、 Feng, Y., Jiao, W., Fu, X., and Chang Z. (2006) "Stepwise disassembly and apparent non-stepwise reassembly for the oligomeric RbsD protein", Protein Science, in press. [PDF]

59、 Zhang, X., Zheng, Y., and Chang, Z. (2006) “Peptide Induced Conformational Changes of E. coli DegP (HtrA) Protease”, Progress in Biochemistry and Biophysics , 33(2):183-189. [PDF]

58 、 Fedurkina, N.V., Belousova, L.V., Mitskevich, L.G., Zhou, H.-M., Chang, Z ., and Kurganov, B.I. (2006) “The change in the kinetic regime of protein aggregation with temperature increase: Thermal aggregation of rabbit muscle creatine kinase”, Biochemistry -Moscow , in press.

*57、 Jiao, W., Li, P., Zhang, J., Zhang, H., Chang, Z . (2005) “Small Heat Shock Proteins Function in the Insoluble Protein Complex”, Biochem Biophys Res Commun ,335(1):227-231. (IP=2.904) [PDF]

*56 、 Chen, X., Fu, X., Ma, Y., and Chang, Z . (2005) “ Chaperone-like activity of Mycobacterium tuberculosis Hsp16.3 does not require its intact (native) structures”, Biochemistry -Moscow,70(8):913-919. (IP=1.058) [PDF]

*55 、 Fu, X. and Chang ,Z. (2005) “Phylogenetic and biochemical studies reveal a potential evolutionary origin of animal small heat shock proteins from bacterial class A” J. Mol. Evol. , in press . (IP=2.751)

*54 、 Hong, W., Jiao, W., Hu, J., Zhang, J., Liu, C., Fu, X., Shen, D., Xia, B., and Chang, Z . (2005) “Periplasmic Protein HdeA Exhibits Chaperone-like Activity Exclusively within Stomach pH Range by Transforming into Disordered Conformation” , J. Biol. Chem., 280(29):27029-27034. (IP=6.355) [PDF]

*53 、 Fu, X. and Chang , Z (2005) “Identification of a highly conserved Pro-Gly in non-animal small heat shock proteins and characterization of its structural and functional roles in Mycobacterium tuberculosis Hsp16.3” Biochemistry -Moscow , 69(5) ： 678-685. (IP=1.058) [PDF]

*52 、 Fu, X., Zhang, H., Zhang, X., Cao, Y., Jiao, W., Liu, C., Song, Y., Abulimiti, A., and Chang, Z. (2005) “A dual role for the N-terminal region of Mycobacterium tuberculosis Hsp16.3 in self-oligomerization and binding denaturing substrate proteins” J. Biol. Chem . , 280 (8) :6337-6348. (IP=6.355) [PDF]

*51 、 Zhang, H. Fu, X. Jiao, W., Zhang, X., Liu, C., and Chang, Z . (2005) “The association of small heat shock protein Hsp16.3 with the plasma membrane of Mycobacterium tuberculosis : dissociation of oligomers is a prerequisite” Biochem Biophys Res Commun , 330:1055-1061. (IP=2.904) [PDF]

*50 、 Jiao ， W., Qian, M., Li, P., Zhao, L., and Chang, Z . (2005) “The essential role of the flexible termini in the temperature-responsiveness of the oligomeric state and chaperone-like activity for the polydisperse small heat shock protein IbpB from Escherichia coli ” J. Mol. Biol . , 347(4):871-884. (IP=5.542) [PDF]

*49 、 Fu, X., Zhang, X., and Chang, Z. (2005) “4'-dianilino-1,1'-binaphthyl-5,5'-sulfonate (bis-ANS), a novel molecule having chaperone-like activity”, Biochem Biophys Res Commun , 329:1087-1093. (IP=2.904) [PDF]

*48 、 Zhang, X.,Fu, X., Zhang, H., Liu, C. Jiao, W., and Chang, Z. (2005) “Chaperone-like Activity of β -Casein”, International Journal of Biochemistry and Cell Biology , 37:1232-1240. (IP=3.578) [PDF]

47、叶子坚，刘冲，陈效友，昌增益 “分枝杆菌中表达重组蛋白的新载体pMSL的构 建”， 江西农业大学学报，2005年第27卷5期，753-758。

*46 、 昌增益，焦旺旺 “细胞内一种耗能蛋白质降解途径的发现： 2004 年诺贝尔化学奖工作介绍”， 生物物理学报 ， 2004 年，第 20 卷第 6 期 , 第 421 － 425 页。

*45 、 陈效友，李传友，马王与，刘冲，王敬慧，张雪峰， 昌增益 “卡介苗菌 MDP1 基因敲除技术的研究” 中国结核和呼吸杂志 ， 2004 年，第 27 卷第 3 期，第 183-187 页。

*44 、 Liu, C. He., Y. and Chang Z . (2004) Truncated hemoglobin of Mycobacterium tuberculosis : The oligomeric state change and the interaction with membrane components” Biochem Biophys Res Commun . 316:1163-1172 . (IP=2.904) [PDF]

*43 、 Liu Y., Fu, X., Shen, J., Zhang, H., Hong, W., and Chang, Z . (2004) “Periplasmic proteins of Escherichia coli are highly resistant to aggregation: A reappraisal for roles of molecular chaperones in periplasm” Biochem Biophys Res Commun . 316(3):795-801. (IP=2.904) [PDF]

*42 、 Fu, X. and Chang, Z . (2004) “Temperature-dependent subunit exchange and chaperone-like activities of Hsp16.3, a small heat shock protein from Mycobacterium tuberculosis ” Biochem Biophys Res Commun . 316:291-299. (IP=2.904) [PDF]

*41 、 Zhang, X. and Chang Z . (2004) “Temperature-dependent protease activity and structural properties of human HtrA2 protease” Biochemistry -Moscow, 69(6):687-692. (IP=1.058) [PDF]

*40 、 Fu, X., Jiao, W., Abulimiti, A. and Chang, Z. (2004) “Inter-subunit cross-linking suppressed the dynamic oligomeric dissociation of Mycobacterium tuberculosis Hsp16.3 and reduced its chaperone activity” Biochemistry -Moscow, 69(5) ： 552-557. (IP=1.058) [PDF]

*39 、 Fu X, Li W, Mao Q, Chang Z . (2003) “Disulfide bonds convert small heat shock protein Hsp16.3 from a chaperone to a non-chaperone: implications for the evolution of cysteine in molecular chaperones” Biochem Biophys Res Commun . 308(3):627-635. (IP=2.904) [PDF]

*38 、 Fu, X. Liu, C., Liu, Y., Feng, X., Gu, L., Chen, X., and Chang, Z. （ 2003 ） “Small Heat Shock Protein Hsp16.3 Modulates Its Chaperone Activity by Adjusting the Rate of Oligomeric Dissociation”. Biochem Biophys Res Commun . 310(2):412-420. (IP=2.904) [PDF]

*37 、 Abulimiti, A., Fu, X., Gu., L., Feng, X., and Chang, Z . (2003) " Mycobacterium tuberculosis Hsp16.3 Nonamers are Assembled and Re-assembled via Trimer and Hexamer Intermediates " J. Mol. Biol . 326(4):1013-1023 . (IP=5.542) [PDF]

*36 、 Abulimiti, A., Qiu, X., Chen, J., Liu, Y., and Chang, Z. (2003) “Reversible methionine sulfoxidation of Mycobacterium tuberculosis small heat shock protein Hsp16.3 and its possible role in scavenging oxidants” Biochem. Biophys. Res. Commun . , 305(1):87-93. (IP=2.904) [PDF]

*35 、 Abulimiti, A. and Chang, Z. (2003) "Alpha-crystallin promotes the assembly of trimeric form of the Mycobacterium tuberculosis Hsp16.3 in cell free system" Biochemistry (Moscow), 68 （ 3 ）： 269-274 . (IP=1.058) [PDF]

34 、 Chen Y, Lu YJ, Wang HW, Quan S, Chang Z , Sui SF. (2003) “Two-dimensional crystallization of a small heat shock protein HSP16.3 on lipid layer” Biochem Biophys Res Commun . 310(2):360-6. [PDF]

*33 、 Gu, L., Abulimiti, A., Li, W., and Chang, Z . (2002) "Monodisperse Hsp16.3 nonamer exhibits dynamic dissociation and reassociation, with the nonamer dissociation prerequisit for chaperone-like activity" J. Mol. Biol . 319 （ 2 ） ： 517-526 . (IP=5.542) [PDF]

32、 Feng, X., Huang, S. Fu, X., Abulimiti, A. and Chang, Z. (2002) "The reassembling process of the nonameric Mycobacterium tuberculosis small heat shock protein Hsp16.3 occurs via a stepwise mechanism" Biochem. J . , 363:329-334 . (IP=4.278) [PDF]

*31 、 毛启龙，冯修光，昌增益 “ 结核杆菌小分子热休克蛋白 Hsp16.3 的高效自发再折叠和再组装 ” 生物化学与生物物理进展 ， 2002 年 ， 29 （ 1 ）： 87-90 。1

*30 、 黄素芳 ， 古良才 ， 毛启龙 ， 昌增益 “ Leu122 对 Hsp16.3 组装过程中亚基相互作用的影响 ” 中国生物化学与分子生物学报 ， 2002 年， 18 （ 1 ）： 99-104 。

29 、 Xiu, Z., Chang, Z., Zeng, A. (2002) "Nonlinear Dynamics of Regulations of Bacterial trp operon: Model Analysis of Integrated Effects of Repression, Feedback Inhibition, and Attenuation" Biochnol. Prog . 18:686-693 （ IP=1.635 ） .

28 、 Pan, G. J., Chang, Z.Y. , Scholer, H.R., and Pei, D. Q. (2002) “Stem cell pluripotency and transcription factor Oct4” Cell Research 12(5-6):321-329. （ IP=1.936 ）

*27 、 Mao, Q., Ke, D., Feng, X. and Chang, Z. (2001) “Preheat Treatment for Mycobacterium tuberculosis Hsp16.3: Correlation Between a Structural Phase Change at 60 o C and a Dramatic Increase in Chaperone-like Activity” Biochem. Biophys. Res. Commun. , 284:942-947 . (IP=2.904) [PDF]

*26 、 Mao, Q., Chang, Z . (2001) “Site-directed Mutation on the only Universally Conserved Residue Leu122 of Small Heat Shock Protein Hsp16.3” Biochem. Biophys. Res. Commun. , 289(5):1257-1261 . (IP=2.904) [PDF]

*25 、 Mao, Q., Ke, D., Chang, Z . (2001) “Electrostatic interaction plays an essential role for Mycotacterium tuberculosis Hsp16.3 to interact with substrate proteins” Biochemistry (Moscow), 66(8):904-908 . (IP=1.058)

*24 、 Mao, Q., Ke, D., and Chang, Z. (2001) "Heat treatment of small heat shock proteins alpha-crystallin and Hsp16.3: Structure changes vs. Chaperone like activity" Tsinghua Science and Technology , 6(5):406-409 .

23 、 Chen, Y., An, J., Ding, Y., Dai, H., Mao, Q., Feng, L., Liu, B., Chang, Y., Chen, F., He, H., Tang, H., Chang, Z. , and Rao, ZH (2001) “Preliminary X-ray crystallographic studies of the Mycobacterium tuberculosis Hsp16.3 molecular chaperone” Protein and Peptide letters , 8(6):499-502.

*22 、 Dai, H., Mao, Q., Yang, H., and Chang, Z . (2000) “Probing the Roles of the Only Universally Conserved Leucine Residue in the Oligomerization and Chaperone-like Activity of Mycobacterium tuberculosis Small Heat Shock Protein Hsp16.3”, J. Protein Chem . , 19(4):319-326 . (IP=1.099) [PDF]

21 、 Wang, L., Duan, M., Zhang, Y. Lin S., and Chang, Z. (2000)“Translocation of P53-regulated laminin receptors in pro-apoptotic microcircustance of human vasculogenesis inhibition”, Cell Biology International , 24(10):745-748

20 、修志龙， 昌增益 ，苏志国 “ 20 世纪生物技术回顾与 21 世纪展望 ” ， 自然杂志 ， 2000 ， 22 （ 219 ）： 233 － 240 。

19 、张代佳，刘传斌，修志龙， 昌增益 “ 微波技术在植物细胞内有效成分提取中的应用 ” ， 中草药 ， 2000 ， 31 （ 9 ）： 5 － 6 。

18 、何秀云，庄玉辉，张晓刚，董恩军，熊志红，昌增益 “ 结核分支杆菌重组 38 000 蛋白抗原诱导豚鼠迟发 性超敏反应的研究 ” ， 中华结核和呼吸杂志 ， 2000 ， 23 （ 3 ）： 161-163 。

17 、熊志红，庄玉辉，张晓刚，何秀云，董恩军，昌增益 “ 重组结核分支杆菌 16 000 蛋白抗原（ rPA16 ）豚鼠 皮肤实验效果的研究 ” 中国现代医学杂志 ， 2000 ， 10 （ 12 ）： 41-44 。

16 、何秀云，庄玉辉，张晓刚，熊志红，董恩军，昌增益 “ 结核分支杆菌重组 38 000 蛋白的免疫原性特征 ” 中华结核和呼吸杂志 ， 2000 ， 23 （ 8 ）： 485-488 。

15 、王琳，段明星，张友忠，孔北华，李少玲，昌增益“纤维粘连蛋白在人滋养上皮的不同部位与功能” 清华大学学报 ， 2000 ， 40 （ 2 ）： 118-121 。

*14 、 Yang, H., Huang, S., Dai, H., Gong, Y., Zheng, C., and Chang, Z . (1999), “The Mycobacterium tuberculosis small heat shock protein HSP16.3 Exposes Hydrophobic Surfaces at Mild Conditions: Conformational Flexibility and Molecular Chaperone Activity”, Protein Science , 8 （ 1 ） :174 － 179. (IP=4.116)

*13 、 杨红梅，毛启龙，薛涛， 昌增益 ， “ 结核杆菌小分子热休克蛋白 Hsp16.3 一高度保守亮氨酸的定点突变研 究 ” ， 清华大学学报（自然科学版） ， 1999 年 36(6):42-45 。

*12 、 昌增益，戴红政，毛启龙，余冰滨 （ 1999 ） “ 克隆的概念，意义与进展 ” 生物学通报 ， 34(11):3-6 。

11 、王琳，段明星，张友忠，孔北华，李少玲，高英茂，昌增益 (1999) “ 层粘连蛋白在正常和异常人滋养上皮的 不同定位与功能 ” 中国组织化学与细胞化学杂志 ， 8(6):366 － 369.

10 、何秀云、庄玉辉、张晓刚、熊志红、昌增益 （ 1999 ） “ 结核分支杆菌 38 000 蛋白质抗原在大肠杆菌中高效 表达 ” 中华结核和呼吸杂志， 22(3):138-141

9 、段名星、庄峰蜂、周志红、张芳、昌增益、郑昌学 （ 1999 ） “ 几种不同基因免疫途径的比较研究 ” ，上海免疫学， 19 （ 5 ）： 275 － 279 。

8 、熊志红，庄玉辉，张晓刚，何秀云，董恩军，昌增益 (1999) “ 结核分子杆菌 16000 抗原基因在大肠杆菌中的 高效表达及其产物纯化 ” 中华结核和呼吸杂志 , 22(11):645-647.

7 、 Chang, Z., Wilson, D.K., Kellems, R.E., and Quiocho, F.A. (1997) “Cysteine not Required for the Catalytic Activity of Adenosine Deaminase” Tsinghua Science and Technology (published in English) , 2(1):441-446.

6 、 Chang, Z., Primm, T.P., Jakana, J., Lee, I.H., Chiu, W., Gilb ert, H.F., and Quiocho, F.A. (1996) “ Mycobacterium tuberculosis 16-kDa Antigen (HSP16.3) Functions as an Oligomeric Structure in Vitro to Suppress Thermal Aggregation” J. Biol. Chem. , 271(12):7218-7223 (IP=6.355)

5 、 Sideraki, V., Mohamedali, K.A.,Wilson, D.K., Chang, Z. , Kellems, R.E., Quiocho, F.A., and Rudolph, F.B. (1996) “Probing the Functional Role of Two Conserved Active Site Aspartates in Mouse Adenosine Deaminase” Biochemistry , 35(4):7862-7872.

4 、 Chang, Z., Choudhary, A. Lathigra, R. and Quiocho, F.A. (1994) “The Immunodominant 38-kDa Lipoprotein Antigen of Mycobacterium tuberculosis Is a Phosphate-binding Protein” J. Biol. Chem. , 269(3):1956-1958. (IP=6.355)

3 、 Chouhary, A., Vyas, M. N. Vyas, N. K. Chang, Z . and Quiocho, F.A. (1994) “Crystallization and Preliminary X-ray Crystallographic Analysis of the 38-kDa Immunodominant Antigen of Mycobacterium tuberculosis ” Protein Science, 3(12):2450-2451.

2 、 Sharff, A. J., Wilson, D. K., Chang, Z. and Quiocho, F. A. (1992) “Refined 2.5 A Structure of Murine Adenosine Deaminase at pH 6.0” J. Mol. Biol. , 226(4):917-921.


1 、 Chang, Z ., Nygaard, P., Chinault, A. C., and Kellems, R. (1991)“Deduced Amino Acid Sequence of Escherichia coli Adenosine Deaminase Reveals Evolutionarily Conserved Amino Acid Residues: Implications for Catalytic Function” Biochemistry, 30(8):2273-2280. (IP=4.008)