Professor with tenure, School of Life Sciences, Peking University; Beijing, China - 2019-Present
Investigator of Beijing Advanced Innovation Center for Genomics (ICG), Peking University; Beijing, China - 2016-Present
Investigator of Peking-Tsinghua Center for Life Sciences, Peking University; Beijing, China - 2015-Present
Investigator of Biomedical Institute for Pioneering Investigation via Convergence (BIOPIC), Peking University; Beijing, China - 2014-Present
Investigator of School of Life Sciences, Peking University; Beijing, China - 2007-Present
Research Associate, Stanford University School of Medicine; Stanford, CA, USA - 2005-2007
Postdoctoral fellow, Stanford University School of Medicine; Stanford, CA, USA - 1999-2004
Honors and Awards
BI Investigatorship Award, 2019
Weiming Outstanding Research Award of the Year 2018 in the School of Life Sciences of Peking
Peking University Award for Advanced Individual in Industry-University-Research Project, 2019
Beijing Science & Technology Award, 2018
China Ministry of Science and Technology Innovation Talent Promotion Plan: Technology Innovation and Entrepreneurship, 2018
Peking University Teaching Award, 2018
China Patent Award, 2016
Tan Jiazhen Life Science Award, 2016
Scientific Chinese Man of the Year, 2015
Zheng Changxue Teaching Award, 2015
Bayer Investigator Award, 2014
Roche Chinese Young Investigator Award, 2014
Peking University Dongbao Fellowship, 2012
Most popular teacher of School of Life Sciences, 2010
Professional Society Affiliations
Academic board member (School of Life Sciences, PKU)
Chair of the scientific seminar committee of CLS
Degree evaluation committee (Life Sciences, PKU)
Interdisciplinary academic advisory committee (PKU)
Judge of the SLS-Qidong Innovation Fund (School of Life Sciences, PKU)
Member of the graduate students education committee (School of Life Sciences, PKU)
Member of Academic Committee of the Key Laboratory of Genome Editing System and
Technology (Ministry of Industry and Information Technology), Harbin Institute of
Member of China Pharmaceutical Registration Review Expert Advisory Committee
Deputy Director, the Division of Genome Editing, Genetics Society of China
Member of a council in the Molecular system biology
Member of China Health Care Association for International Exchange of Birth Defects
Precision Medicine Branch
Member of a council in the Chinese Association of Biochemistry (Beijing)
Section Editor (Genome Editing and New Technology) for SCIENCE CHINA Life Sciences
Our laboratory is interested in studying the molecular mechanism of human diseases, especially the host response to microbial pathogenicity. The combination of forward and reverse genetic means is employed, often in a high-throughput fashion, for the identification of host genes of interest. Efforts have also been put into the development and application of eukaryotic gene editing techniques, such as TALENs and CRISPR/Cas9 systems.
Representative Peer-Reviewed Publications
Qu L, Yi Z, Zhu S, Wang C, Cao Z, Zhou Z, Yuan P, Yu Y, Tian F, Liu Z, Bao Y, Zhao Y, Wei W*. (2019) Program-mable RNA editing by recruiting endogenous ADAR using engineered RNAs. Nat. Biotechnol. doi: s41587-019-0178-z.
X. Zhao, G. Zhang, X. Chen, L. Dai, X. Qu, S. Li, S. Liu, H. Song, Z. Gao, P. Yuan, Z. Liu, C. Li, Z. Shang, Y. Li, M. Zhang, J. Qi, H. Wang, N. Du, Y. Wu, Y. Bi, S. Gao, Y. Shi, J. Yan, Y. Zhang, Z. Xie*, W. Wei* and G. F. Gao*. (2019) Human Neonatal Fc Receptor Is the Cellular Uncoating Receptor for Enterovirus B. Cell 177, 1-13.
Zhu S, Cao Z, Liu Z, He Y, Wang Y, Yuan P, Li W, Wei W*. (2019) Guide RNAs with embedded barcodes boost CRISPR-pooled screens. Genome Biol 20, 20, doi:10.1186/s13059-019-1628-0.
Liu Y, Cao Z, Wang Y, Guo Y, Xu P, Yuan P, Liu Z, He Y, and Wei W*. (2018) Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotechnol 36, 1203-1210.
Zhang H, Zhou Y, Wang Y, Zhao Y, Qiu Y, Zhang X, Yue D, Zhou Z, and Wei W*. (2018). A surrogate reporter sys-tem for multiplexable evaluation of CRISPR/Cas9 in targeted mutagenesis. Sci Rep 8, 1042.
Zhang Y, Liu L, Guo S, Song J, Zhu C, Yue Z, Wei W* and Yi C*. (2017) Deciphering TAL effectors for 5-methylcytosine and 5-hydroxymethylcytosine recognition. Nat Commun 8(1): 901.
He R, Peng J, Yuan P, Yang J, Wu X, Wang Y and Wei W*. (2017). Glucosyltransferase Activity of Clostridium dif-ficile Toxin B Triggers Autophagy-mediated Cell Growth Arrest. Sci Rep 7(1): 10532.
Zhou Y, Wang P, Tian F, Gao G, Huang L*, Wei W* and Xie X.S* (2017) Painting A Specific Chromosome with CRISPR/Cas9 for Live-cell Imaging. Cell Res. 27, 298-301.
Hu H, Zhang H, Wang S, Ding M, An H, Hou Y, Yang X, Wei W*, Sun Y*, Tang C*. (2017) Live visualization of ge-nomic loci with BiFC-TALE. Sci. Rep. 7, 40192.
Zhou Y, Zhang H and Wei W*. (2016) Simultaneous generation of multi-gene knockouts in human cells. FEBS Letters 590, 4343-4353.
Zhu S, Li W, Liu J, Chen C-H, Liao Q, Xu P, Xu H, Xiao T, Cao Z, Peng J, Yuan P, Brown M, Liu X* & Wei W*. (2016) Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR library. Nat Biotechnol 34, 1279-1286.
Peng J, Zhou Y, Zhu S and Wei W*. (2015) High-throughput Screens in Mammalian Cells Using the CRISPR-Cas9 System. FEBS Journal 282, 2089–2096.
He R, Peng J, Yuan P, Xu F, Wei W*. (2015) Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy 11:5, 740-747.
Ren Q, Li C, Yuan P, Cai C, Luo G, Zhang L and Wei W*. (2015) A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus. Sci. Rep. 5, 8865; DOI:10.1038/srep08865.
Shen J, Cai C, Yu Z, Pang Y, Zhou Y, Qian L, Wei W* and Huang Y*. (2015) A microfluidic live cell assay to study anthrax toxin induced cell lethality assisted by conditioned medium. Sci. Rep. 5, 8651; DOI:10.1038/srep08651.
Yuan P, Zhang H, Cai C, Zhu S, Zhou Y, Yang X, He R, Li C, Guo S, Li S, Huang T, Feng H and Wei W*. (2015) Chondroitin sulfate proteoglycan 4 functions as the cellular receptor for Clostridium difficile toxin B. Cell Res. 25:157-168.
Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y and Wei W*. (2014) High-throughput Screening of a CRISPR/Cas Li-brary for Functional Genomics in Human Cells. Nature 509(7501): 487-91.
Qian LL, Cai CZ, Yuan PF, Jeong SY, Yang XZ, Almeida VD, Ernst J, Costa M, Cohen SN, Wei WS*. (2014) Bidirec-tional effect of Wnt signaling antagonist DKK1 on the modulation of anthrax toxin uptake. Sci China Life Sci, 57: 469–481.
Yang J, Zhang Y, Yuan P, Cai C, Ren Q, Guo S, Zhu C, Qi H and Wei W*. (2014) Complete Decoding of DNA Recognition by TAL Effector RVDs. Cell Res. 24:628-631.
Yang J, Yuan P, Wen D , Sheng Y, Zhu S, Yu Y, Gao X and Wei W*. (2013) ULtiMATE system for rapid assembly of customized TAL effectors. PLoS One 2013; 8:e75649.
research is mainly focused on the development of eukaryotic genome editing
tools, especially their applications in high-throughput functional genomics.
The combination of forward and reverse genetic means is employed, often in a
high-throughput fashion, for the identification of host genes important for
host response during microbial infections or tumorigenesis.