Dr. Shi-Qiang Wang received his B.S. and Ph.D. degrees from College of Life Sciences, Peking University, China in 1990 and 1998, respectively. In 2003, he completed postdoctoral research in National Institute on Aging, NIH, USA, and retuned to Peking University. In 2005, he was honored as a Cheung Kong Scholar Professor by the Ministry of Education. Dr. Wang’s studies have been focused on the molecular basis of intracellular Ca2+ signaling. He combines state-of-the-art confocal imaging, single-channel patch clamping, single-molecule tracing with molecular cell biology techniques to dissect the molecular mechanisms underlying the generation, amplification and termination of intracellular Ca2+ dynamics, aiming to answer important questions about the function, development and dysfunction of the heart. Many of his work were published in high profile journals, including Nature, PLoS Biology, PNAS and Circulation Research. He gained a number of nationally and internationally-recognized awards and prizes, such as the Chang Hsi-chun Young Investigator Prize by Chinese Association for Physiological Sciences (1997), the Richard J. Bing Young Investigator Award by International Society for Heart Research (2001), the Katz Basic Science Research Prize by American Heart Association (2002),and the National Natural Science Prize of China (2017).
1999.1 - 2003.11, Postdoctoral fellow and Research Associate, National Institute on Aging, NIH
1992.9 - 1998.7, Ph.D., Physiology, Peking University
1986.9 - 1990.7, B.S., Physiology & Biophysics, Peking University
2003.8 - present, Professor, College of Life Sciences, Peking University
1999.7 - 2003.7, Associate Professor, College of Life Sciences, Peking University
1994.7 - 1999.6, Lecturer, College of Life Sciences, Peking University
1990.8 - 1994.6, Assistant Lecturer, Department of Biology, Peking University
Molecular mechanism of calcium signaling
Ca2+ is a universal intracellular messenger, playing pivotal roles in nearly all biological processes. We combine state-of-the-art molecular imaging, single-channel patch clamping, electron microscopy and molecular cell biology techniques to dissect the molecular mechanisms underlying the generation, amplification and termination of intracellular Ca2+ dynamics, aiming to answer important questions about the function, development and dysfunction of heart cells.
Representative Peer-Reviewed Publications
1. Yang L, Li RC, Xiang B, Li YC, Wang LP, Guo YB, Liang JH, Wang XT, Hou T, Xing X, Zhou ZQ, Ye H, Feng RQ, Lakatta EG, Chai Z, Wang SQ* (2021) Transcriptional regulation of intermolecular Ca2+ signaling in ground squirrel cardiomyocytes: the myocardin- junctophilin axis. Proc. Natl. Acad. Sci. U.S.A. 2021;118:/e2025333118. Doi:10.1073/pnas.2025333118.
2. Li LL, Guo QJ, Luo HY, Liang JH, Yang Y, Xing X, Li HT, Han J, Shen S, Li H, Ye HH, Wu HD, Cui BX*, Wang SQ* (2020) Nanobar Array Assay Revealed Complementary Roles of BIN1 Splice Isoforms in Cardiac T-Tubule Morphogenesis. Nano Letters, 20(9):6387-6395.
3. Yang HQ, Zhou P, Wang LP, Zhao YT, Ren YJ, Guo YB, Xu M, Wang SQ* (2020) Compartmentalized β1-adrenergic signaling synchronizes excitation-contraction coupling without modulating individual Ca2+ sparks in healthy and hypertrophied cardiomyocytes. Cardiov. Res., 116:2069-2080.
4. Yang HQ, Wang LP, Gong Y, Fan XX, Zhu SY, Wang XT, Wang YP, Li LL, Xing X, Liu XX, Ji GS, Hou T, Zhang Y, Xiao RP, Wang SQ* (2019) β2-Adrenergic Stimulation Compartmentalizes β1 Signaling into Nanoscale Local Domains by Targeting the C-Terminus of β1-Adrenoceptors. Circ. Res., 124:1350-1359.
5. Zhao YT, Guo YB, Gu L, Fan XX, Yang HQ, Chen Z, Zhou P, Yuan Q, Ji G, Wang SQ (2017) Sensitized signaling between L-type Ca2+ channels and ryanodine receptors in the absence or inhibition of FKBP12.6 in cardiomyocytes. Cardiov. Res. 113:332-342.
6. Xiao E, Yang H, Gan YH, Duan DH, He LH, Guo Y, Wang SQ*, Zhang Y* (2015) TRPM7 Senses Mechanical Stimulation Inducing Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells. Stem Cells, 33:615-621.
7. Shang W, Lu F, Sun T, Xu J, Li LL, Wang Y, Wang G, Chen L, Wang X, Cannell MB, Wang SQ*, Cheng H* (2014) Imaging Ca2+ Nanosparks in Heart with a New Targeted Biosensor. Cir. Res. 114:412-420.
8. Li RC, Tao J, Guo YB, Wu HD, Liu RF, Bai Y, Lv ZZ, Luo GZ, Li LL, Wang M, Yang HQ, Gao W, Han QD, Zhang YY, Wang XJ, Xu M, Wang SQ* (2013) In Vivo Suppression of MicroRNA-24 Prevents the Transition Toward Decompensated Hypertrophy in Aortic-Constricted Mice. Circ. Res. 112:601-605.
9. Zhang HB, Li RC, Xu M, Xu SM, Lai YS, Wu HD, Xie XJ, Gao W, Ye H, Zhang YY, Meng X, Wang SQ* (2013) Ultrastructural Uncoupling between T-tubules and Sarcoplasmic Reticulum in Human Heart Failure. Cardiov. Res. 98:269-276.
10. Xu M, Wu HD, Li RC, Zhang HB, Wang M, Tao J, Feng XH, Guo YB, Li SF, Lai ST, Zhou P, Li LL, Yang HQ, Luo GZ, Bai Y, Xi JZ J., Gao W, Han QD, Zhang YY, Wang XJ*, Meng X, and Wang SQ* (2012) MiR-24 regulates junctophilin-2 expression in cardiomyocytes. Circ. Res. 111:837-841.
11. Wu HD, Xu M, Li RC, Guo L, Lai YS, Xu SM, Li ll, Lü QL, Zhang HB, Zhang YY, Zhang CM and Wang SQ* (2012) Ultrastructural Remodeling of Ca2+ Signaling Apparatus in Failing Heart Cells. Cardiov. Res. 95:430-438.
12. Zhou P, Zhao YT, Guo YB, Xu SM, Bai SH, Lakatta EG, Cheng H, Hao XM, Wang SQ* (2009) Beta-Adrenergic signaling accelerates and synchronizes cardiac ryanodine receptor response to a single L-type Ca2+ channel. Proc. Natl. Acad. Sci. U. S. A. , 106(42):18028-18033.
13. Tang AH, Wang SQ* (2009) Transition of spiral calcium waves between multiple stable patterns can be triggered by a single calcium spark in a fire-diffuse-fire model. Chaos, 19(3):037114.
14. Xu M, Zhou P, Xu SM, Liu Y, Feng XH, Bai SH, Bai Y, Hao XM, Han QD, Zhang YY*, Wang SQ* (2007) Intermolecular Failure of L-type Ca2+ Channel and Ryanodine Receptor Signaling in Hypertrophy. PLoS Biology, 5(2): e21,0203-0211.
15. Zhou C, Tai C, Ye HH, Ren X, Chen JG, Wang, SQ*, Chai Z* (2006) Interleukin-1b downregulates the L-Type Ca channel activity by depressing the expression of channel protein in cortical neurons. Journal of Cellular physiology, 206(3):799-806.
16. Fu Y, Zhang GQ, Hao XM, Wu CH, Chai Z, Wang SQ* (2005) Temperature dependence and thermodynamic properties of Ca2+ sparks in rat cardiomyocytes. Biophysical Journal, 89(4):2533-2541.
17. Wang SQ, Stern MD, Ríos E, Cheng H (2004) The quantal nature of Ca2+ sparks and in situ operation of the ryanodine receptor array. Proc. Natl. Acad. Sci. U. S. A. , 101(11): 3979-3984.
18. Liao XD, Tang AH, Chen Q, Jin HJ, Wu CH, Chen LY, Wang SQ* (2003) Role of Ca2+ signaling in initiation of stretch-induced apoptosis in neonatal heart cells. Biochemical and Biophysical Research Communications, 310(2):405-411.
19. Wang SQ, Song LS, Xu L, Meissner G, Lakatta EG, Rios E, Stern MD, Cheng H* (2002) Thermodynamically irreversible gating of ryanodine receptors in situ revealed by stereotyped duration of release in Ca2+ sparks. Biophysical Journal, 83(1): 242-251.
20. Wang SQ ,Song LS, Lakatta EG, Cheng H (2001) Ca2+ signaling between single L-type Ca2+ channels and ryanodine receptors in heart cells. Nature, 410(6828):592-596.