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HU, Jiazhi
Title:
Investigator
Lab Phone: 44611
Lab Address: LUI CHE WOO BUILDING,Peking University, No.5 Yiheyuan Road, Haidian District,Beijing, P.R.China 100871
Lab Homepage: http://hulab.pku.edu.cn
Personal Homepage: http://
Resume
Education
Sep. 2006 –Aug. 2012, College of Life Sciences, Peking University, Beijing, China
Ph. D. in Biochemistry and Molecular Biology. Supervisor: Dr. Daochun Kong.

Sep. 2002 - July 2006, College of Life Sciences, Peking University, Beijing, China
B.S. in Biotechnology.
Professional Experience
Oct. 2016 – present, Center for Life Sciences and College of Life Sciences, Peking University
Assistant professor

Sep. 2012 – Sep. 2016, Program in Cellular & Molecular Medicine, Boston Children’s Hospital, Boston, MA, USA
Research Fellow in Dr. Frederick Alt’s lab.
Honors and Awards
2017 Youth 1000 Talents Plan
2016 Harvard Chinese Life Science Research Award
2014 Cancer Research Institute Irvington Fellowship
2012 Ray Wu Prize
2012 Beijing Outstanding Graduate Student
2012 Excellent Graduate Thesis of Peking University
2012 Outstanding Graduate of Peking University
2012 Leica Top Grade Scholarship
2012 Johnson & Johnson Asia Outstanding Graduate Thesis Award in Bio-tech
Research Interests
Lymphocyte receptors, including B cell receptor/antibody and T cell receptor, show high diversity during generation and high specificity during antigen recognition. Lymphocyte receptor-mediated adaptive immune system can efficiently eliminate pathogens, but genome instability can also convert normal lymphocytes into lymphomas or leukemia. My major research interest is immunogenomics and human diseases, to be more specific, tumors. Immunogenomics refers to a new branch of immunology that relies on genomics analyzing methods to study important immune processes. Immunogenomics reinforces our knowledge about adaptive immunity, the biggest resource of human genetic variations.

Aim 1. To study the regulatory mechanism during generation of diverse lymphocyte receptors.

Aim 2. To study the mechanism of antigen and lymphocyte receptor recognition.

Aim 3. To study the development of lymphoid tumors and screen for neoantigen for cancer therapy.

Aim 4. To study the genome instability during genome editing.
Representative Peer-Reviewed Publications
SELECTED PUBLICATIONS (# co-first authors, *co-corresponding authors)

Zuo E#, Huo X#, Yao X#, Hu X#, Sun Y#, Yin J#, He B, Wang X, Shi L, Ping J, Wei Y, Ying W, Wei W, Liu W, Tang C, Li Y, Hu J* & Yang H*. (2017). CRISPR/Cas9-mediated targeted chromosome elimination. Genome Biology 18(1):224. doi: 10.1186/s13059-017-1354-4.

Lin SG#, Ba Z#, Du Z#, Zhang Y, Hu J* & Alt FW*. (2016). A highly sensitive and unbiased approach for elucidating antibody repertoires. Proc Natl Acad Sci USA 113(28):7846-51. doi:10.1073/pnas.1608649113.

Hu J#, Meyers RM#, Dong J, Panchakshari RA, Alt FW* & Frock RL*. (2016). Detecting DNA double-stranded breaks in mammalian genomes by linear amplification-mediated high-throughput genome-wide translocation sequencing. Nature Protocols 11(5): 853-71. doi: 10.1038/nprot.2016.043.

Hu J#, Zhang Y#, Zhao L, Frock RL, Du Z, Meyers RM, Meng F-L, Schatz DG, & Alt FW. (2015). Chromosomal loop domains direct the recombination of antigen receptor genes. Cell 163(4): 947-59. doi: 10.1016/j.cell.2015.10.016.

Frock RL#, Hu J#, & Alt FW. (2015). Mechanisms of recurrent chromosomal translocations. In book: Chromosomal Translocations and Genome Rearrangements in Cancer. Springer International Publishing, ISBN: 978-3-319-19983-2. doi: 10.1007/978-3-319-19983-2

Frock RL#, Hu J#, Meyers RM, Ho Y-J, Kii E, & Alt FW. (2015). Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases. Nature Biotechnology 33(2):179-86. doi: 10.1038/ nbt.3101.

Hu J#, Tepsuporn S#, Meyers RM, Gostissa M*, & Alt FW*. (2014). Developmental propagation of V(D)J recombination-associated DNA breaks and translocations in mature B cells via dicentric chromosomes. Proc Natl Acad Sci USA 111(28): 10269-74. doi: 10.1073/pnas.1410112111.

Tepsuporn S#, Hu J#, Gostissa M*, & Alt FW*. (2014). Mechanisms that can promote peripheral B-cell lymphoma in ATM-deficient mice. Cancer Immunol. Res. 2(9): 857-66. doi: 10.1158/2326-6066. CIR-14-0090.

Hu J, Sun L, Shen F, Chen Y, Hua Y, Liu Y, Zhang M, Hu Y, Wang Q, Xu W, Sun F, Ji J, Murray JM, Carr AM, & Kong D. (2012). The intra-S phase checkpoint pathway targets Dna2 to prevent stalled replication forks from reversing. Cell 149(6): 1221-32. doi: 10.1016/j.cell.2012.04.030.



OTHER PUBLICATIONS (# co-first authors, *co-corresponding authors)

Guan L, He P, Yang F, Zhang Y, Hu Y, Ding J, Hua Y, Zhang Y, Ye Q, Hu J, Wang T, Jin C, Kong D. (2017). Sap1 is a replication initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe. J Biol Chem. 292(15):6056-6075. doi: 10.1074/jbc.M116.767806.

Liu B, Hu J, Wang J, Kong D. (2017). Direct visualization of RNA-DNA primer removal from Okazaki Fragments provides support for flap cleavage and exonucleolytic pathways in eukaryotic cells. J Biol Chem. 292(15):6056-6075. doi: 10.1074/jbc.M116.758599.

Zhao L#, Frock RL#, Du Z, Hu J, Cheng L, Krangel MS*, Alt FW*. (2016). Orientation-specific RAG activity in chromosomal loop domains contributes to Tcr V(D)J recombination during T cell development. J Exp Med 213(9): 1921-36. doi: 10.1084/jem.20160670.

Dong J#, Panchakshari RA#, Zhang T#, Zhang Y, Hu J, Volpi SA, Meyers RM, Ho Y-J, Du Z, Robbiani DF, Meng F-L, Gostissa M, Nussenzweig MC, Manis JP*, & Alt FW*. (2015). Orientation-specific joining of AID-initiated DNA breaks promotes antibody class switching. Nature 525(7567): 134-9. doi: 10.1038/nature14970.

Meng F-L#, Du Z#, Federation A#, Hu J, Wang Q, Kieffer-Kwon KR, Meyers RM, Amor C, Wasserman CR, Neuberg D, Casellas R, Nussenzweig MC, Bradner JE*, Liu XS, & Alt FW*. (2014). Convergent transcription at intragenic Super-Enhancers targets AID-initiated genomic instability. Cell 159(7): 1538-48. doi: 10.1016/j.cell. 2014.11.014.
Teaching
Immunology
Laboratory Introduction
Immunogenomics and human diseases. Immunogenomics refers to a new branch of immunology that relies on genomics analyzing methods to study important immune processes. Immunogenomics reinforces our knowledge about adaptive immunity, the biggest resource of human genetic variations.
Laboratory Phone:62744611