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Xiaofeng Zheng, Ph.D.

Professor, School of Life Science, Peking University

tel : 86 - 10 - 6275-5712

fax : 86 - 10 - 6275-5913

New Life Science Building, Peking University, Summer Palace Road No. 5, Beijing, P. R. China 100871

Research Interests

Our lab is interested in three major research fields:

1. Functional and structural studies on human diseases (especially cancer and inflammation) related proteins.
2. Mechanism of protein-RNA interaction.
3. Antivirals on influenza virus and mechanism of preS antigen in HBV infection.

The study of novel proteins with important biological functions is of particular interests to us. To identify and further understand the structures and functions of novel proteins will help in the prediction of protein structures and the discovery of functional relationships of proteins to their structures, as well as protein engineering of industrial enzymes and structure-based drug design. Our targets include the proteins related to human diseases and some novel proteins with functions previously unknown. In addition to determine the crystal structures of these proteins, we take molecular, biochemical and cellular approaches to investigate the biological roles of these proteins, to understand the molecular mechanisms of protein-protein interactions, the biological pathways involved and their relationships to human diseases.

Publications

• Meng G, Bai X, Green TJ, Luo M, Zheng XF*, Crystallization and preliminary X-ray crystallographic studies on SI-CLP, a novel human Glyco_18 domain–containing protein , Protein & Peptide Letter, 2009 ,In press.
• Chen B, Lian M, Luo M, Zheng XF, A chemical lipid modification of recombinant preS antigen to study the mechanism of HBV attachment to the host cell , J Biotechnology, 2008 ,137: 8-13.
• Zhao YM, Zhang JF, Li HY, Li YY, Ren J, Luo M, Zheng XF , An NADPH sensor protein (HSCARG) downregulates nitric oxide synthesis by association with argininosuccinate synthetase and is essential for epithelial cell viability. , J Biol. Chem. , 2008 , 283(16):11004-11013. , This paper has been selected by Science Signaling as Editor's Choice (Citation: N. R. Gough, NADPH Sensor Regulates NO Production. Sci. Signal. 1, ec142 (2008).) , [Abstract] , [Full Text]
• Meng G, Zhai RT, Liu B, Zheng XF , Identification of a novel nuclear-localized adenylate kinase from Drosophila melanogaster. , Biochemistry (Moscow). , 2008 , 73: 38-43.
• Lian M, Zhou X, Chen B, Li C, Gu X, Luo M, Zheng XF , Identification of the critical regions in HBV preS required for its stability. , J Peptide Science. , 2008 , 14(3): 307-312. , [Abstract] , [Full Text]
• Meng G, Bai X, Green TJ, Luo M, Zheng XF , Crystallization and preliminary X-ray crystallographic studies on SI-CLP, a novel human Glyco_18 domain–containing protein , Protein & Peptide Letter , 2008 , In press
• Chen B, Lian M, Luo M, Zheng XF , A chemical lipid modification of recombinant preS antigen to study the mechanism of HBV attachment to the host cell. , J Biotechnology , 2008 , In press
• Nallagatla SR, Hwang J, Toroney R, Zheng XF, Cameron CE, Bevilacqua PC , 5'-triphosphate-dependent activation of PKR by RNAs with short stem-loops. , Science , 2007 , 318: 1455-1458. , [Abstract] , [Full Text]
• Zheng XF, Dai XY, Zhao YM, Chen Q, Lu F, Yao DQ, Liu XP, Zhang CM, Gu X, Luo M , Restructuring of the dinucleotide binding fold in a NADP(H) sensor protein. , Proc.Natl.Acad.Sci.USA. , 2007 , 104(21): 8809-8814. , [Abstract] , [Full Text]
• Chen Q, You DL, Liang YH, Su XD, Gu X, Luo M, Zheng XF , Crystal structure of the Thermoanaerobacter tengcongensis hypoxanthine-guanine phosphoribosyltransferase L160I mutant: insights into the inhibitor design. , FEBS J. , 2007 , 274: 4408-4415. , [Abstract] , [Full Text]
• Li CY, Yu Q, Ye ZQ, Sun Y, He QY, Li XM, Zhang WX, Luo JC, Gu X, Zheng XF, Wei LP , A Nonsynonymous SNP in Human Cytosolic Sialidase in a Small Asian Population Results in Reduced Enzyme Activity: Potential Link with Severe Adverse Reactions to Oseltamivir. , Cell Research. , 2007 , 17(4):357-362. , [Abstract] , [Full Text]
• Lian M, Zhou X, Wei L, Qiu SH, Zhou T, Li L, Gu X, Luo M, Zheng XF , 3. Serum levels of preS antigen (HBpreSAg) in chronic hepatitis B virus infected patients , Virology J. , 2007 , 4: 93. , [Abstract] , [Full Text]
• Chen Q, Hui R, Sun C, Gu X, Luo M, Zheng XF , Soluble expression, purification, and stabilization of a pro-apoptotic human protein, CARP. , Protein Expression & Purification. , 2006 , 45(2):329-334.
• Yu YM, Li L, Zheng XF, Liang YH, Su XD , Protein Preparation, Crystallization and Preliminary X-ray Analysis of Imidazolonepropionase from Bacillus subtilis. , BBA - Proteins and Proteomics , 2006 , 1764, 153-156.
• Dai X, Gu X, Luo M, Zheng XF , Protein expression, crystallization and preliminary X-ray crystallographic studies on HSCARG from Homo sapiens. , Protein & Peptide Letter. , 2006 , 13(9):955-957.
• Zhai RT, Meng G, Zhao YM, Liu B, Zhang GF, Zheng XF , A novel nuclear-localized protein with special adenylate kinase properties from Caenorhabditis elegans. , FEBS Letters. , 2006 , 580: 3811-3817. , [Abstract]
• Ren H, Wang LY, Bennett M, Liang YH, Zheng XF, Lu F, Li LF, Luo M, Eriksson S,. Zhang CM, Su XD , The Crystal Structure of human AK6: An Adenylate Kinase Isoform Localized to the Cell Nucleus. , Proc.Natl.Acad.Sci.USA. , 2005 , 102 (2):303-8.
• Dai X, Chen Q, Lian M, Zhou Y, Zhou M, Lu SY, Chen YJ, Luo JC, Gu X, Jiang Y, Luo M, Zheng XF , Systematic high-yield production of human secreted proteins in Escherichia coli. , Biochem Biophys Research Commun. , 2005 , 332: 593-601.
• Li D, Liang YH, Zheng XF, Li L, Su XD , Protein preparation, Crystallization and Preliminary X-ray Analysis of YjcG protein from Bacillus subtilis. , Acta Cryst. Sect. F , 2005 , Volume 61（5）Epub
• Chen Q, Liang YH, Su XD, Gu X, Zheng XF, Luo M , Novel IMP Binding in Feedback Inhibition of Hypoxanthine-Guanine Phosphoribosyltransferase from Thermoanaerobacter tengcongensis. , J. Mol. Biol. , 2005 , 348(5):1199-1210.
• Ren H, LiangYH, Li R, Ding H, Qiu S, Lu S, An J, Li L, Luo M, Zheng XF, Su XD , Protein preparation, Crystallization and Preliminary X-ray Analysis of human adrenal gland protein AD-004. , Acta Cryst D , 2004 , 60: 1292-1294.
• Zheng XF and Bevilacqua PC , A novel class of activators of PKR: small double-stranded RNAs with single-stranded tails. , RNA , 2004 , 10:1934-1945. , [Abstract] , [Full Text]
• Gong W, Liu H, Niu L, Shi Y, Tang Y, Teng M, Wu J, Liang D, Wang D, Wang J, Ding J, Hu H, Huang Q, Zhang Q, Lu S, An J, Liang Y, Zheng X, Gu X, Su XD , Structural genomics efforts at the Chinese Academy of Sciences and Peking University. , J Struct Funct Genomics. , 2003 , 4(23): 137-139.
• Zheng XF and Bevilacqua PC. , Efficient construction of long DNA duplexes with internal non-Watson-Crick motifs and modifications. , Nucleic. Acids Res. , 2001 , 29(2) e6.
• Zheng XF and Bevilacqua PC. , Straightening of bulged RNA by the double-stranded RNA-binding domain from the protein kinase PKR. , Proc.Natl.Acad.Sci.USA , 2000 , 97(26):14162-14167. , [Abstract] , [Full Text]
• Zheng XF, Kobayashi Y, Takeuchi M. , Construction of a low serine-type carboxypeptidase producing mutant of Aspergillus oryzae by the expression of antisense RNA and its use as a host for heterologous protein secretion. , Appl. Microbiol. Biotechnology , 1998 , 49:39-44.
• Zheng XF and Huang BQ , Marker proteins for somatic embryogenesis in several plants. , Acta Bontanica Sinica , 1994 , 36(3):175-180.
• Zheng XF and Huang BQ , Intranuclear and cytoplasmic inclusions in embryogenic and embryoidal cells of Panax quinquefolius. , Acta Bontanica Sinica , 1994 , 35(6): 422-428.
• Wang L, Zheng XF, Zhang G , Rapid propagation of Leymus giganteus L. , Plant Physiology Communications , 1990 , 3:47-48.

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